The 2PM TM is an ultracompact, low-price two-photon microscope for life cell and multiphoton deep tissue imaging. Autofluorescence, Second Harmonic Generation (SHG), fluorescent proteins, and exogeneous fluorophores can be detected. Optical sectioning is achieved by multiphoton excitation and xy-galvoscanning and piezo-driven focusing optics.
Transfected cancer cells expressing GFP in the nuclei and DsRed in the cytoplasm
Multiple exposure modes can be applied: (i) region of interest (ROI) scanning, (ii) single point illumination, and (iii) line scan. Additionally the 2PM TM microscope can be equipped with a Fluorescence Lifetime Imaging (FLIM) module based on Time-Correlated Single Photon Counting (TCSPC). The 2PM TM is computer-controlled and includes JenLab Scan software and SPCImage software for data acquisition and image processing.