11th Workshop on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques FLIM 2017 June 30 - July 1st, Berlin, Germany
Compact Cryo Two-Photon Microscope
High submicron resolution imaging during freezing and heating based on near infrared femtosecond laser technology
-196°C - +600°C (77K – 873K) cooling rates: 0.01K/min - 150K/min • Label-free autofluorescence of frozen samples • Fluorescence Lifetime Imaging (FLIM) • Second Harmonic Generation (SHG) • Microspectroscopy Applications: Cryopreservation, thermostress, climate change, optimization of cryogenic protocols, High Tech tool for cryobanks, Human, animal, plant tissue/ cells/ minerals
Two photon cryo FLIM microscopy of plant leaves (Arabidopsis thaliana). Chlorophyll fluorescence in intratissue chloroplasts. High spatial and temporal resolution (300 nm / 200 ps).
• compact turn-key tunable Ti:Sapphire femtosecond laser laser pulse width: 100 fs - 200 fs repetition frequency: 80 MHz laser power: < 1.3 W wavelength range. 710-920 nm • full-frame scanning, region-of-interest (ROI) scanning, line scanning, single-point illumination (spot scan) • typical FOV: 250 μm x 250 μm (horizontal); WD: < 2 mm • typical spatial resolution: < 0.5 μm (horizontal); < 2 μm (vertical) • typical temporal resolution: 200 ps (TCSPC, up to 256 time channels) • focusing optics: 40x NA 1.3 (standard), other objectives possible • control and image processing software (JenLab Control, JenLab Image) • temperature range -196°C (nitrogen) - +600°C (77K - 873K) • cooling rates: 0.01K/min - 150K/min • power requirements: 230 VAC (50 Hz) or 115 VAC (60 Hz) • CE certified • 700x520x800mm³ (without laser) Notes: These specifications are subject to change without notice. Reference: Breunig, Tümer, König. Multiphoton imaging of freezing and heating effects in plant leaves. J Biophotonics (2012), in press
Summary of the 2PM™-Cryo in Adobe PDF format (click here, 1,4 MB)